Description
The Plasmid DNA Maxi Kit uses the convenient spinning column format to deliver high-quality plasmid DNA following a simple “bind-wash-elute” procedure without the need for expensive accessories. Yields vary according to plasmid copy number, E. coli strain, and growth conditions. 50-200 ml of bacterial cultures in LB medium normally produce 0.6-1.2 mg of plasmid DNA with a high copy number. Up to 500 ml of culture can be processed when working with a low copy number plasmid.
The system uses centrifugation or vacuum technology for plasmid purification and eliminates time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads greater than 800 bp), restriction enzyme digestion, ligation, PCR, in vitro transcription, transformation, and other applications.
Features
Speed: plasmid DNA purification in <60 minutes
Safety: no phenol / chloroform extractions
Versatile: spin and vacuum formats available
High quality – DNA is suitable for a variety of downstream applications
Specifications
- Subsequent application Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription, etc.
- Starting material: 50-200 mL LB culture with OD600 between 2 and 3; or the equivalent
- Plasmid type: Cosmid DNA high copy, low copy
- Processing mode: Manual (centrifugation or vacuum)
- Performance: 1 – 24
- Binding technology: Silica Maxi Spin Column DNA
- Lysate removal method: Centrifugation
- Processing time: <60 minutes
- Yield: 600-1200 µg for high copy number; 50-300 µg for low copy number
