Evaluation of the multiplex real-time PCR assays RealStar malaria S&T

Posted on October 1, 2020Categories cDNA, Culture Cells, Panel, PCR, Pcr Kits, Ria Kits, RNA, Vector & VirusTags , , , , , , , , , , , , , , , , , , , ,

GABA enzymatic assay package. We developed an enzymatic assay system enabling simple quantification of 4-aminobutyric acid (GABA). The response of GABA aminotransferase obtained from Streptomyces decoyicus NBRC 13977 was mixed to these of the beforehand developed glutamate assay system utilizing glutamate oxidase and peroxidase. The three-enzyme system permitting GABA-dependent dye formation as a result of oxidative coupling between 4-aminoantipyrine and Trinder’s reagent enabled correct quantification of 0.2 – 150 mg/L GABA. A pretreatment combination consisting of glutamate oxidase, ascorbate oxidase and catalase eliminating glutamate, … Continue reading “Evaluation of the multiplex real-time PCR assays RealStar malaria S&T”